M-HeLa

Origin: human, epithelioid cervical carcinoma, clone from HeLa. J. Exp. Med. 1953, 97: 695,

Cytology (Rus.) 1986, 28: 562.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - BS 10 %

subculture procedure - cells detachment using EDTA 0.04 %, split ratio 1 : 5

cryoconservation - growth medium, 30 % BS, 5 % DMSO,

 1.0 - 1.5x106 cells/ml in ampule.

Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: modal number of chromosomes 51, number of markers - 12-17.

Other properties:

virus susceptibility: adenoviruses, poliovirus, RS, parainfluenza.

Applications: cell biology, virology, biotechnology (test systems preparation,

 accumulation of viral mass)

Collections: SPBII.

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