Origin: human, epithelioid cervical carcinoma, clone from HeLa. J. Exp. Med. 1953, 97: 695,
Cytology (Rus.) 1986, 28: 562.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - BS 10 %
subculture procedure - cells detachment using EDTA 0.04 %, split ratio 1 : 5
cryoconservation - growth medium, 30 % BS, 5 % DMSO,
1.0 - 1.5x106 cells/ml in ampule.
Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: modal number of chromosomes 51, number of markers - 12-17.
virus susceptibility: adenoviruses, poliovirus, RS, parainfluenza.
Applications: cell biology, virology, biotechnology (test systems preparation,
accumulation of viral mass)