Origin: human, embryo, lung.
Fed.Proc. 1960. 19: 386.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - BS 20%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3-1:5, optimal population density 1.5-1.8x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 2.0õ106 cells/ml in ampule
Viability after cryoconservation: 70% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=46, variability in the range between 54-138 chromosomes,
modal number of chromosomes 65-70.
Plating efficiency: 60% (ESCC)
virus susceptibility: polioviruses, human adenoviruses, RSV.
Collections: ATCC CCL 5; ECACC 89111004; ESCC.