L132

Origin: human, embryo, lung.

Fed.Proc. 1960. 19: 386.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - BS 20%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3-1:5, optimal population density 1.5-1.8x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0106 cells/ml in ampule

Viability after cryoconservation: 70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 54-138 chromosomes,

modal number of chromosomes 65-70.

Plating efficiency: 60% (ESCC)

Other properties:

virus susceptibility: polioviruses, human adenoviruses, RSV.

Applications: virology.

Collections: ATCC CCL 5; ECACC 89111004; ESCC.

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