L-41-M

Origin: human, bone marrow of a leukemic subject, clone from J 96 line. Blood 1955, 10: 1010.

Tsitologia i Genetika 1981, 15: 68-73.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - BS 10 %

subculture procedure - cells detachment using EDTA 0.04 %, split ratio 1 : 3

cryoconservation - growth medium, 30 % BS, 5 % DMSO,

1.0 - 1.5x106 cells/ml in ampule.

Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Other properties:

virus susceptibility: vesicular stomatitis, RS, parainfluenza type 3, adeno-, lymphocytic

choriomeningitis, herpes simplex.

Applications: cell biology, virology, interferone titration, biotechnology (preparation

of test systems, accumulation of viral mass).

Collections: SPBII, SPBIC.

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