Origin: human, acute myelogenous leukemia (bone marrow)

Science 1978. 200: 1153-1154; Blood 1980. 56: 344-350; Blood 1979. 54: Suppl. 1, 174a.

Morphology: myeloblastoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640 or Iscove's DMEM

serum - FBS 20%

subculture procedure - optimal population density 3.0-9.0105 cells/ml

cryoconservation - growth medium, 5% DMSO, 2.5106 cells/ml in ampule

Viability after cryoconservation: 85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46, variability in the range between 44-49 chromosomes, modal number

of chromosomes 46-47, number of markers - 5 (differential dye) (ATCC).

Plating efficiency: the cells cannot be plated (ATCC)

Tumorigenicity: non tumorigenic

Other properties:

isoenzymes G6PD, B; PGM1,1; PGM3, 0; ES D, 1; Me-2, 1; AK 1,0; GLO-1,2.

Have no surface Ig antigens.

Erythrocyte rosette tests: E, 0; EA, 2%; EAC, 0.

HLA cell line phenotype 30, 31; 35; Cw 4.

Express the human DR antigen.

Differentiation into non-dividing macrophages when exposed to phorbol esters; formation

of colonies in soft-agar culture when exposed to colony-stimulating factor

Applications: tumorigenicity, differentiation

Collections: ATCC CCL 246; DSM ACC 14; ECACC 86111306; SPBIC.