Origin: human, oral epidermoid carcinoma, subline of KB.
Mode of cultivation: monolayer
Conditions for cultivation: medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,
split ratio 1:4-1:10, optimal population density 0.5-1.0õ105 cells/cm2
cryoconservation - medium 199 70%, BS 20%, glycerol 10%, 2.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation: 70-80% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 60-73 chromosomes, modal number
of chromosomes 66, number of markers - 4 specific for Hela - N 1, 2, 3, 4, G-banding),
number of polyploid cells 6.0%.
virus susceptibility: polioviruses; adenoviruses; arboviruses; rhinoviruses; measles; Coxsackie;
ECHO; vaccinia; influenza; herpes simplex.
Isoenzymes G6PD, A.
This line probably was contaminated with Hela cells.
Presence of oncornavirus B (ESCC).
Applications: virology, tumorigenicity.
Collections: MWIIW; ESCC.