KBv

Origin: human, oral epidermoid carcinoma, subline of KB.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:4-1:10, optimal population density 0.5-1.0105 cells/cm2

cryoconservation - medium 199 70%, BS 20%, glycerol 10%, 2.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 70-80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 60-73 chromosomes, modal number

of chromosomes 66, number of markers - 4 specific for Hela - N 1, 2, 3, 4, G-banding),

number of polyploid cells 6.0%.

Other properties:

virus susceptibility: polioviruses; adenoviruses; arboviruses; rhinoviruses; measles; Coxsackie;

 ECHO; vaccinia; influenza; herpes simplex.

Isoenzymes G6PD, A.

This line probably was contaminated with Hela cells.

Presence of oncornavirus B (ESCC).

Applications: virology, tumorigenicity.

Collections: MWIIW; ESCC.

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