KB

Origin: human, oral epidermoid carcinoma

Proc.Soc.Exp.Biol.Med. 1955. 89: 362; Proc.Soc.Exp.Biol.Med. 1956. 91: 361;

Proc.Soc.Exp.Biol.Med. 1957. 94: 661; Cancer Res. 1958. 18: 1017; Science 1961. 133: 1559.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:3 - 1:10, optimal population density 1.0-3.0104 cells/cm2

cryoconservation - growth medium, 5-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 63-84 chromosomes, modal number

of chromosomes 73-75, number of markers - 25 (differential dye).

Plating efficiency: 64% (ATCC)

Other properties:

virus susceptibility: poliovirus 1-3, adenovirus 3, 7; herpes simplex, influenza, vaccinia; Coxsackie B1, B3, B5.

Applications: tumorigenicity, virology, biochemistry

Collections: : ATCC CCL 17; ECACC 86103004; ICLC HTL 96014; SPBIC.

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