K-562

Origin: human, chronic myelogenous leukemia (pleural effusion).

Blood 1975. 45: 321-334; J.Natl.Cancer Inst. 1977. 59; 77;

Int.J.Cancer 1979. 23: 143-147; Leukemia Res. 1979. 3; 363;

Proc. 37th Ann.Meet.Electron Microsc.Soc.Amer., tex. 1979: 234;

Blood 1980. 56: 344-350; J.Biol.Chem. 1980. 255: 3266;

Biochem.J. 1981. 193: 361; Proc.Soc.Exp.Biol.Med. 1981. 166: 546-550;

J.Immunol. 1982. 129; 2504; Exp.Hematol. 1983. 11: 601-610;

Clin.Haemotol.1984.13:461; Biology of the cell in culture. L. Nauka,1984.279.

Morphology: erythromyeloblastoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 1.0x105-1.0x106 cells/ml

cryoconservation - growth medium (may add 40% FBS), 10%

DMSO, 3.0-7.0106 cells/ml in ampule

Viability after cryoconservation: 93% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, there are some sublines K-562 with different karyotypic structure.

One from sublines is: variability in the range between 55-69 chromosomes,

modal number of chromosomes 64, number of markers - 12 (differential dye), number of polyploid cells 3%.

Plating efficiency: the cells cannot be plated (ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

haemoglobin synthesis.

Isoenzymes AK 1,1; ES D,1; GLO-1, 2; G6PD, B; PGM1, 0; PGM3,1; Me-2,0.

Erythrocyte rosette tests: E, 1%; EA, 34%; EAC, 2%.

Capable to differentiate into progenitors of the erythrocytic, granulocytic and monocytic series.

Not contained B- and T-markers.

Applications: differentiation, cell biology, natural killer assay, pharmacodynamics.

Collections: ATCC CCL 243; ECACC 89121407; DSM ACB 10; ICLC HTL 94001; MWIIW; SPBII; SPBIC.

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