Origin: human, T-lymphoblastic leukemia

Submitted from Institute of Immunology, Moscow.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 3.0-9.0x105 cells/ml

cryoconservation - growth medium, 10% DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 41-49 chromosomes,

modal number of chromosomes 46-47, number of markers - 2 (differential dye).

Other properties:

IL-2 synthesis, T-cell marker CD 3.

Applications: immunology, biochemistry, differentiation

Collections: SPBIC.