Origin: human, T-lymphoblastic leukemia
Submitted from Institute of Immunology, Moscow.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 3.0-9.0x105 cells/ml
cryoconservation - growth medium, 10% DMSO, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 41-49 chromosomes,
modal number of chromosomes 46-47, number of markers - 2 (differential dye).
IL-2 synthesis, T-cell marker CD 3.
Applications: immunology, biochemistry, differentiation