Origin: human, neuroblastoma
Cancer Res. 1970. 30: 2110.
Morphology: fibroblast- and neuroblast-like
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM or DMEM
serum - FBS 10%
other components - NEAA 1% (EMEM)
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 2.0-3.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.5-2.0õ106 cells/ml in ampule
Viability after cryoconservation: 76% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 42-51 chromosomes, modal number o
f chromosomes 49, number of markers - 2 (differential dye), number of polyploid cells 16%.
Plating efficiency: less than 1% (ATCC)
Tumorigenicity: tumorigenic in nude mice
virus susceptibility: vesicular stomatitis (Indiana), herpes simplex, vaccinia, adenovirus 12, Coxsackie B3.
Isoenzymes G6PD, B;
Applications: tumorigenicity, immunology, differentiation, electrophysiology, cell biology
Collections: ÀÒÑÑ CCL 127: ECACC 86041809; ICLC HTL 96021; SPBIC.