Origin: human, neuroblastoma

Cancer Res. 1970. 30: 2110.

Morphology: fibroblast- and neuroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS 10%

other components - NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 2.0-3.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.5-2.0106 cells/ml in ampule

Viability after cryoconservation: 76% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 42-51 chromosomes, modal number o

f chromosomes 49, number of markers - 2 (differential dye), number of polyploid cells 16%.

Plating efficiency: less than 1% (ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: vesicular stomatitis (Indiana), herpes simplex, vaccinia, adenovirus 12, Coxsackie B3.

Isoenzymes G6PD, B;

neurotransmitter synthesis.

Applications: tumorigenicity, immunology, differentiation, electrophysiology, cell biology

Collections: CCL 127: ECACC 86041809; ICLC HTL 96021; SPBIC.