IM-9

Origin: human, bone marrow, myeloma

Ann NY Acad.Sci. 1972. 190: 221-234; PNAS 1974. 71: 84-88; Nature 1974. 251: 443-444;

J.Biol. Chem. 1974. 249: 1661-1667; J.Biol. Chem. 1976. 251: 6844-6851.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 2.0-4.0x105 cells/ml

cryoconservation - growth medium, 10% DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 43-48 chromosomes, modal number of

chromosomes 46, normal human karyotype (46, XX), (ATCC) number of polyploid cells 55%.

Plating efficiency: the cells cannot be plated ( ATCC)

Other properties:

isoenzymes PGM1,1-2; PGM3, 0; ES T-D,1; Me-2, 2; GLO-1,1-2; G6PD, B.

Human growth hormone receptor, insulin receptor, calcitonin receptor.

Erythrocyte rosette tests: E, 1%; EA, 0; EAC, 13%.

Applications: biotechnology (Ig G kappa production), endocrinology, Tumorigenicity:

Collections: ATCC CCL 159; DSM ACC 117; ECACC 86051302; SPBIC.

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