HT-29

Origin: human, colon adenocarcinoma.

"Human tumor cells in vitro", NY, London, Plenum Press. 1975. 115; J.Natl.Cancer Inst. 1977. 58: 209;

J.Natl.Cancer Res. 1977. 59: 221; 1988. 48; 6193; Tissue Antigens 1978. 11: 279.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

 split ratio 1:3-1:5, optimal population density 1.0105 cells/cm2

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 87% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 67-73 chromosomes,

modal number of chromosomes 70,  number of markers - 2 large submetacentric and metacentric

chromosomes (routine dye), number of polyploid cells 2.0%.

Other properties:

virus susceptibility: vesicular stomatitis.

Isoenzymes G6PD, B; Me-2, 1; PGM3, 2-1; FUC, V2-1; PGM1, V2-1; ESD, 1; AK1, 1; PGD, A.

HLA cell line phenotype A (1/11, 3); B (12, 17); Ek-1; Ek-11.

Applications: virology, tumorigenicity.

Collections: ATCC HTB 38; ECACC 91072201; MWIIW.

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