Origin: human, kidney hypernephroma.
Biolog.Nauki 1985, 6: 29.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME or EMEM
serum - BS10% or FBS 2.5%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3) or EDTA 0.04 %,
split ratio 1:2 - 1:3.
cryoconservation - BME, 20-30% BS, 5-10% DMSO, 1.0-1.5õ106 cells/ml in ampule
Viability after cryoconservation: 75% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n=46 , variability in the range between 55-74 chromosomes, modal number of chromosomes 62.
Tumorigenicity: produce tumors in the cheek pouch of the hamster
virus susceptibility: vesicular stomatitis, herpes simplex, cytomegalovirus, adenoviruses, RSV,
encephalomyocarditis, parainfluenza 1 and 2, SV-40.
Applications: biochemistry, immunology, cell biology, virology.