HN

Origin: human, kidney hypernephroma.

Biolog.Nauki 1985, 6: 29.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME or EMEM

serum - BS10% or FBS 2.5%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3) or EDTA 0.04 %,

split ratio 1:2 - 1:3.

cryoconservation - BME, 20-30% BS, 5-10% DMSO, 1.0-1.5106 cells/ml in ampule

Viability after cryoconservation: 75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46 , variability in the range between 55-74 chromosomes, modal number of chromosomes 62.

Tumorigenicity: produce tumors in the cheek pouch of the hamster

Other properties:

virus susceptibility: vesicular stomatitis, herpes simplex, cytomegalovirus, adenoviruses, RSV,

encephalomyocarditis, parainfluenza 1 and 2, SV-40.

Applications: biochemistry, immunology, cell biology, virology.

Collections: SPBIC.

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