HL-60

Origin: human, peripheral blood, promyelocytic leukemia.

Nature 1977. 270: 347-349; Blood 1979. 54: 713-733; Cytology (Russ.) 1992. 34: 123.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640 (Initial growth is sometimes by using Iscove's DMEM)

serum - FBS 20%

subculture procedure - split ratio 1:2, optimal population density 1.0-5.0x105 cells/cm2

cryoconservation - growth medium, 5%DMSO, 3.0-5.0x106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46 , variability in the range between 43-47 chromosomes, modal number of chromosomes 45,

number of markers - 6 (differential dye), double minute chromosomes, number of polyploid cells 3%.

Plating efficiency: : the cells cannot be plated ( ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: HIV-1, HTLV-1.

Isoenzymes G6PD, B; PGM1,1; PGM3,1; ES D,1; Me-2,1; AK 1,1; GLO-1,1.

Erythrocyte rosette tests: E, 4%; EA, 17%; EAC, 1%.

Applications: differentiation, pharmacodynamics, Tumorigenicity:

Collections: : ATCC CCL 240; ECACC 88112501; DSM ACC 3; ICLC HTL 95010; SPBIC.

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