Hep G2

Origin: human, hepatocyte carcinoma

Nature 1979. 282: 615-616; Science 1980. 209: 497-499.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%, sodium pyruvate 0.1%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:3 - 1:6, optimal population density 2.0-3.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46 , variability in the range between 49-57 chromosomes, modal number of chromosomes 55,

number of polyploid cells - 5.6%.

Tumorigenicity: non tumorigenic in nude mice

Other properties:

produce a -fetoprotein, albumin, a 2-macroglobulin, a 1-antitrypsin, transferrin, a 1-antichymotrypsin, haptoglobin,

 ceruloplasmin, plasminogen, complement (C3, C4), C3 activator, fibrinogen, a 1-acid glycoprotein,

a 2-HS glycoprotein, b -lipoprotein, retinol binding protein.

Applications: biotechnology, biochemistry, virology, receptor study, enzymology, differentiation, cell biology

Collections: ATCC HB 8065; ECACC 85011430; SPBIC.