Hep-2v

Origin: human, larynx, epidermoid carcinoma, subline Hep-2.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199 or EMEM.

serum - BS 10% (199) or FBS 10% (EMEM).

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:4 -1:10, optimal population density 0.5-1.0105 cells/cm2

cryoconservation - medium - 199 70% + BS 20% or EMEM 70% + FBS 20%,

glycerol 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation: 88% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 40-64 chromosomes, modal number of chromosomes 58-59,

number of markers - 13 (3 - specific for Hela - N 1, 3, 4, G-banding), number of polyploid cells 8.0%.

Tumorigenicity: tumorigenic

Other properties:

virus susceptibility: polioviruses; adenoviruses; vesicular stomatitis; measles; herpes simplex; Coxsackie; ECHO;

parotitis; Newcastle disease; arboviruses; RSV.

Isoenzymes G6PD, A.

This line probably was contaminated with Hela cells.

Applications: virology.

Collections: MWIIW.

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