HEp-2

Origin: human, epidermoid larynx carcinoma

Cancer Res. 1954. 14: 660; Cancer Res. 1955. 15: 598; Proc.Soc.Exp.Biol.Med. 1956. 93: 107.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME or EMEM or DMEM

serum - FBS or BS 10%

other components - NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3) or EDTA 0.04 %,

split ratio 1:3 - 1:10, optimal population density 1.0-3.0x104 cells/cm2

cryoconservation - growth medium (may add 30% BS), 5-10% DMSO, 1.0-1.5106 cells/ml in ampule

Viability after cryoconservation: 80-88% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46 , variability in the range between 68-77 chromosomes, modal number of chromosomes 69-70,

number of markers - 32 (differential dye)

Plating efficiency: 44% (ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: adenovirus 3, herpes simplex, poliovirus 1, RSV, vesicular stomatitis (Indiana),

arbovirus, measles, Coxsackie, ECHO virus, Newcastle disease virus, paramyxoviruses 2 and 3.

Isoenzymes G6PD, A

Applications: virology , biotechnology (virus titration), Tumorigenicity: , cytotoxicity.

Collections: ATCC CCL 23; ECACC 86030501; MWIIW; ESCC; SPBIC; SPBII.

Up