HUMAN CELL LINES

Origin: human, amnion, subline of FL.

Proc.Soc.Exp.Biol.Med. 1957. 94: 532; Cancer Res. 1958. 18: 692;

 J.Natl.Cancer Inst. 1959. 23: 893; Arch. Gesamte Virusforsch. 1960. 9: 559; Proc.Soc.Exp.Biol.Med. 1965. 119: 223.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199 or EMEM

serum - BS 10% (199) or FBS 10% (EMEM)

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:4 -1:10, optimal population density 0.5-1.0105 cells/ml

cryoconservation - medium - 199 70%+BS 20% or EMEM 70%+ FBS 20%,

glycerol 10%, 4.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 65% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 47-66 chromosomes, modal number of chromosomes 60,

 number of markers - 9 (3 - specific for Hela - N 1, 2, 3, G-banding).

Plating efficiency: 85% (ESCC)

Other properties:

virus susceptibility: poliovirus 1; adenoviruses; reoviruses; rhinoviruses; Coxsackie; measles; ECHO;

vaccinia; RSV, parainfluenza.

Isoenzymes G6PD, A.

Susceptibility to human interferon.

This line probably was contaminated with Hela cells.

Presence of oncoviruses A and B (ESCC).

Applications: virology, tumorigenicity, cell biology.

Collections: MWIIW; ESCC.

Up