Origin: human, embryo, lung.
In the book "Epidemiology, Clinic and Prophylactic of Virus Infection", Ekaterinburg, 1992. 45.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:2-1:3,
optimal population density 1.4-1.6x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 2.0õ106 cells/ml in ampule
Viability after cryoconservation: 70% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 45-49 chromosomes, modal number of chromosomes 46.
Plating efficiency: 60%
virus susceptibility: TBE virus; HSV; CMV; RSV; human adenoviruses.
Finite lifetime culture, cells are capable of attaining 49-50 population doubling before onset
of the decline in proliferation.
Applications: virology, biotechnology.