FLECH

Origin: human, embryo, lung.

In the book "Epidemiology, Clinic and Prophylactic of Virus Infection", Ekaterinburg, 1992. 45.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:2-1:3,

optimal population density 1.4-1.6x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0106 cells/ml in ampule

Viability after cryoconservation: 70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 45-49 chromosomes, modal number of chromosomes 46.

Plating efficiency: 60%

Other properties:

virus susceptibility: TBE virus; HSV; CMV; RSV; human adenoviruses.

Finite lifetime culture, cells are capable of attaining 49-50 population doubling before onset

 of the decline in proliferation.

Applications: virology, biotechnology.

Collections: ESCC

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