Daudi

Origin: human, Burkitt lymphoma.

Cancer Res. 1968. 28: 1300; J. Gen. Virology 1976. 33: 539; Clin. Exp. Immunol. 1976. 25: 367;

Int.J.Cancer 1977. 19: 334; Tissue Antigens 1978. 11: 96; Proc.Natl.Acad.Sci. 1978. 75: 3846.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 2.0-5.0 cells/ml

cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%,

5.0-10.0106 cells/ml in ampule

Viability after cryoconservation: 72-84% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 43-48 chromosomes,

modal number of chromosomes 46,

pseudodiploid, Y-chromosome is presented (routine and differential dye, C-banding).

Plating efficiency: the cells cannot be plated ( ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: non susceptible to the Semliki Forest virus infection.

Isoenzymes G6PD, B.

Exhibit surface markers for the Fc fragment of Ig G, complement receptors and surface bound Ig.

The cells are negative for HLA-A and HLA-B antigens.

Positive for genetical markers EBV, EBNA and VCA.

High sensitive to the depressed mitotic activity of interferon.

Applications: virology, tumorigenicity.

Collections: ATCC CCL 213; DSM ACC 78; ECACC 85011437; 89120702;

 ICLC HTL 95024; MWIIW.

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