Caco-2

Origin: human, colon adenocarcinoma

J. Natl.Cancer Inst. 1977. 58: 209-214; J. Natl.Cancer Inst. 1977. 59: 221-226.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS 10-15%

other components - NEAA 1%, sodium pyruvate 0.1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:1 - 1:3), split ratio 1:3 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 5-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 80% ( 0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis (ATCC)

Karyology: 2n=46, variability in the range between 91-107 chromosomes,

modal number of chromosomes 96-101, number of markers - 10 (differential dye),

number of poliploid cells 3.2% (ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

isoenzymes Me-2,1; PGM3,1; PGM1, 1; ES D,1; AK 1,1; GLO-1,1; G6PD, B.

Lipid production.

Applications: gastroenterology, biochemistry, tumorigenicity, cell biology, biophysics.

Collections: ATCC HTB 37; ECACC 86010202; SPBIC.

 

Up