BT-474

Origin: human, breast, ductal carcinoma

J.Natl.Cancer Inst. 1978. 61: 967-978; In vitro 1979. 15: 723-729.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

other components - bovine insulin 10 m /ml

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:4

cryoconservation - growth medium 10% DMSO, 5.0๕106 cells/ml in ampule

Viability after cryoconservation: 71 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46, variability in the range between 95-107 chromosomes, modal number of chromosomes 100-103,

number of markers - 1 large submetacentric chromosome (routine dye), and 9 markers (differential dye, ATCC),

 number of poliploid cells 0.2%

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: mouse mammary tumor virus R-III-MuMTV;

isoenzymes G6PD, B; PGM1,1; PGM3,1; ES D,1; Me-2, 0; AK1, 1; GLO-1,1;

R-III-MuMTV replication.

Applications: tumorigenicity, virology, cell biology

Collections: ภาัั อาย 20; SPBIC.

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