549

Origin: human, lung carcinoma

J.Natl.Cancer Inst. 1973. 51: 1417-1423; Int.J.Cancer 1976. 17: 62-70;

Tissue Antigens 1978. 11:279.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F12K, DMEM, BME or EMEM

serum - FBS or BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:3 -1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium (may add 30% BS), 5-10% DMSO or glycerol,

1.0-1.5106 cells/ml in ampule

Viability after cryoconservation: 97% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 55-68 chromosomes,

modal number of chromosomes 62-65 ,

number of markers - 1 large submetacentric chromosome (routine dye),

number of poliploid cells - 3.2%

Plating efficiency: 48% ()

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: adenovirus, herpes simplex, parainfluenza II and III, polioviruses,

cytomegalovirus, vesicular stomatitis.

High specific activities of choline kinase and cholinephosphate cytidyl-transferase.

Fatty acids synthesis (lecitine).

Interleukine-6 synthesis, interferon receptors.

HLA cell line phenotype F (10,w19); B (8,12).

Applications: biotechnology (interferon induction and titration), tumorigenicity, cell biology,

enzymology, virology

Collections: ATCC CCL 185; ECACC 86012804; MWIIW; SPBII; SPBIC.

 

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