A 431

Origin: human, epidermoid carcinoma

&#J.Natl.Cancer Inst. 1973. 51: 1417-1423.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (3:7), split ratio 1:3 - 1:6,

optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 83% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 55-77 chromosomes, modal number of chromosomes 68-71

Tumorigenicity: tumorigenic in anti-thymocyte serum - treated NIH/Swiss mice.

Other properties:

large numbers of EGF binding sites

Applications: cell biology, growth factors study

Collections: ATCC CRL 1555; ECACC 85090402; SPBIC.