Origin: human, epidermoid carcinoma
J.Natl.Cancer Inst. 1973. 51: 1417-1423.
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (3:7), split ratio 1:3 - 1:6,
optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 83% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=46, variability in the range between 55-77 chromosomes, modal number of chromosomes 68-71
Tumorigenicity: tumorigenic in anti-thymocyte serum - treated NIH/Swiss mice.
large numbers of EGF binding sites
Applications: cell biology, growth factors study
Collections: ATCC CRL 1555; ECACC 85090402; SPBIC.