Origin: human, rhabdomyosarcoma.
Tissue Antigens 1978. 11: 279.
Mode of cultivation: monolayer /suspension
Conditions for cultivation: medium - EMEM
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,
split ratio 1:3, optimal population density 1.0-2.0x105 cells/ml
cryoconservation - EMEM 70%, FBS 20%, glycerol 10%, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 76% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH and G6PD) analysis
Karyology: 2n= 46, variability in the range between 81-95 chromosomes, modal number of chromosomes 87, number of markers - 2 (differential dye - C-banding), specific Hela marker chromosomes not detected ( G banding), number of polyploid cells 2.0%.
virus susceptibility: oncornaviruses in particular leucoviruses.
Isoenzymes G6PD,B; PGM 3,1; PGM 1,1.
HLA cell line phenotype A (1,2); B (8,14); C (w3).
Applications: virology, tumorigenicity, biochemistry.