A-204v

Origin: human, rhabdomyosarcoma.

Tissue Antigens 1978. 11: 279.

Morphology: epithelial-like

Mode of cultivation: monolayer /suspension

Conditions for cultivation: medium - EMEM

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:3, optimal population density 1.0-2.0x105 cells/ml

cryoconservation - EMEM 70%, FBS 20%, glycerol 10%, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 76% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology: 2n= 46, variability in the range between 81-95 chromosomes, modal number of chromosomes 87, number of markers - 2 (differential dye - C-banding), specific Hela marker chromosomes not detected ( G banding), number of polyploid cells 2.0%.

Tumorigenicity: tumorigenic

Other properties:

virus susceptibility: oncornaviruses in particular leucoviruses.

Isoenzymes G6PD,B; PGM 3,1; PGM 1,1.

HLA cell line phenotype A (1,2); B (8,14); C (w3).

Applications: virology, tumorigenicity, biochemistry.

Collections: MWIIW.

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