A-172

Origin: human, glioblastoma

J.Natl.Cancer Inst. 1973. 51: 1417-1423.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:2 - 1:8 , optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 98 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, modal number of chromosomes 80, number of markers - 20 (differential dye) (ATCC)

Tumorigenicity: non-tumorigenic in anti-thymocyte serum - treated NIH/Swiss mice.

Applications: tumorigenicity

Collections: ATCC CRL 1620; ECACC 88062428; SPBIC.

 

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