Origin: human, glioblastoma
J.Natl.Cancer Inst. 1973. 51: 1417-1423.
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:2 - 1:8 , optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 98 % (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, modal number of chromosomes 80, number of markers - 20 (differential dye) (ATCC)
Tumorigenicity: non-tumorigenic in anti-thymocyte serum - treated NIH/Swiss mice.
Collections: ATCC CRL 1620; ECACC 88062428; SPBIC.