ZR-75-1

Origin: human, mammary gland carcinoma (ascitic effusion)

Cancer Res. 1978. 38: 3352-3364 4327-4339.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

other components - sodium pyruvate 1mM

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:1), split ratio 1:4 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 6.0106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 55-77 chromosomes, modal number

of chromosomes 72, number of markers - 18 (differential dye, ATCC), number of polyploid cells 0.8%.

Other properties:

receptors for estrogen and other steroid hormones.

Applications: tumorigenicity, cell biology.

Collections: ATCC CRL 1500; ECACC 87012601; SPBIC.

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