Origin: human, mammary gland carcinoma (ascitic effusion)
Cancer Res. 1978. 38: 3352-3364 è 4327-4339.
Mode of cultivation: monolayer
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
other components - sodium pyruvate 1mM
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:1), split ratio 1:4 - 1:6, optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 6.0õ106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, variability in the range between 55-77 chromosomes, modal number
of chromosomes 72, number of markers - 18 (differential dye, ATCC), number of polyploid cells 0.8%.
receptors for estrogen and other steroid hormones.
Applications: tumorigenicity, cell biology.
Collections: ATCC CRL 1500; ECACC 87012601; SPBIC.