WI-38 VA 13 subline 2RA

Origin: human, embryonic lung, an SV 40 virus-transformed derivative of the WI-38 cell line.

Ann.Med.Exp.Biol.Fenn.1966. 44:242; J.Natl.Cancer Inst.1964. 32: 917.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or BME with twice the concentration of amino acids and vitamins.

serum - FBS 10%

other components - NEAA 1%, sodium pyruvate 1mM (EMEM).

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 1.0-3.0104 cells/cm2

cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 80-85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 45-89 chromosomes, modal number of chromosomes 73-78, number of markers - 2-3 (routine dye), 1-6 microchromosomes (ATCC)

Plating efficiency: 15% (ATCC).

Other properties:

virus susceptibility: herpes simplex, vesicular stomaitits (Indiana), poliovirus 2, reovirus 3.

Isoenzymes G6PD.

Contains SV 40 neo (T) and transplantation antigens.

Applications: biochemistry, transformation, virology.

Collections: ATCC CCL 75.1; ECACC 85062512; SPBIC.