U-251 MG

Origin: human, glioma

Med.Biol. 1978. 56: 184.

Morphology: glial

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:5

cryoconservation - growth medium, 10% DMSO,

1.0x106 cells/ml in ampule

Viability after cryoconservation: 98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 39 -52 chromosomes,

modal number of chromosomes 48, 50, without markers (routine dye), number of polyploid cells 1,4%

Applications: cell biology, tumorigenicity.

Collections: SPBIC