Origin: human, glioma
Med.Biol. 1978. 56: 184.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation: 98% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, variability in the range between 39 -52 chromosomes,
modal number of chromosomes 48, 50, without markers (routine dye), number of polyploid cells 1,4%
Applications: cell biology, tumorigenicity.