Origin: human, uterine leiomyosarcoma.
J. Natl.Cancer Inst. 1977. 59: 221-226; Cancer Genet. Cytogenet. 1988, 33: 77-81
Mode of cultivation: monolayer (weak adhesion)
Conditions for cultivation: medium - EMEM
serum - FBS 10%
other components - NEAA 1%, sodium pyruvate 1mM
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5
cryoconservation - growth medium, 8%DMSO, 1.0-2.0х106 cells/ml in ampule
Viability after cryoconservation: 82% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, variability in the range between 44-48 chromosomes, modal number
of chromosomes 46, normal human karyotype (46, XX), number of polyploid cells 0.6%.
Tumorigenicity: tumorigenic in nude mice
isoenzymes Me2,1-2; PGM3,1; PGM1,1; ESD,1; AK 1,1; GLO-1,1-2; G6PD,B
Applications: tumorigenicity, cytogenetics, cell biology.
Collections: ATCC HTB 115; SPBIC