Origin: human, neuroblastoma (metastasis to supra-orbital area)

Cancer Res. 1973. 33: 2643; In Vitro 1973. 8: 410; Cancer Res. 1977. 37: 1364.

Morphology: epithelial-like and neuroblast-like

Mode of cultivation: monolayer 

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1% 

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:6 - 1:12

cryoconservation - growth medium, 5-10% DMSO, 6.0-9.0х106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis 

Karyology: 2n= 46, variability in the range between 44-47 chromosomes, modal number

of chromosomes 46, pseudodiploid, number of markers - 10 (differential dye) (ATCC)

Tumorigenicity: tumorigenic: produce neuroblastoma in nude mice; produce tumors

in the cheek pouch of the hamster.

Other properties:

isoenzymes Me-2,2; PGM3,1-2; PGM1,1; ES D,2; AK-1,1; GLO-1,1-2; G6PD,B.

Catecholamine production.

Applications: neurophysiology, biochemistry.

Collections: ATCC HTB 10; SPBIC.