SK-HEP-1

Origin: human, liver adenocarcinoma (ascitic fluid).

J.Natl.Cancer Inst. 1977. 58: 209; J.Natl.Cancer Inst. 1977. 59: 221.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%, sodium pyruvate 1mM.

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:2 - 1:4, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 93% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 58-64 chromosomes, modal number

of chromosomes 60-61, number of markers - 8 (differential dye, ATCC), 50% of cells have

large acrocentric chromosome, number of polyploid cells 0.4%.

Tumorigenicity: tumorigenic in nude mice

Other properties:

isoenzymes Me-2, 1-2; PGM3,1; PGM1, 2; ES D,1; AK 1,1; GLO-1,1; G6PD,B.

bFGF production.

Applications: tumorigenicity:

Collections: ATCC HTB 52; ECACC 91091816; SPBIC.

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