RPMI 8226

Origin: human, myeloma

Proc.Soc.Exp.Biol.Med. 1967, 125: 1246-1250

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10-20%

subculture procedure - optimal population density 5.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 8.8x106 cells/ml in ampule

Viability after cryoconservation: 55% (0 passage, dye trypan blue)

Sterility: bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46 variability in the range between 57-73 chromosomes, modal number

of chromosomes 68-70, number of markers - 2 (routine dye ) ()

Plating efficiency: the cells cannot be plated ()

Other properties:

virus susceptibility: poliovirus 1, vesicular stomatitis (Indiana Strain), herpes simplex, vaccinia.

Isoenzymes G6PD, .

Secrete l -type light chains of immunoglobulin.

Erythrocyte rosette tests: E, 0; EA, 1%; EAC, 13%.

HLA cell line phenotype : Aw 19, B 15, B 37, Cw 2.

Applications:cell biology, tumorigenicity: , immunology, biotechnology (production Ig)

Collections: ATCC CCL 155, ECACC 87012702; SPBIC.

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