RPMI 2650

Origin: human, nasal septum carcinoma (Pleural effusion)

Cancer 1964. 17: 170; Exp. Cell Res. 1965. 39: 190.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:1),

split ratio 1:2 - 1:6, optimal population density 2.0-4.0x104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation: 85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and immunofluorescent analysis

Karyology: 2n= 46, variability in the range between 44-46 chromosomes, modal number

of chromosomes 46, pseudodiploid, number of markers - 4 (differential dye).

Plating efficiency: 2% (SPBIC)

Other properties:

virus susceptibility: poliovirus 1, herpes simplex, vesic. stomatitis (Indiana).

Isoenzymes G6PD, B.

Mucopolysaccharide production

Applications: tumorigenicity, cell biology.

Collections: ATCC CCL 30; ECACC 88031602; SPBIC.

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