Origin: human, leukocytes of peripheral blood from healthy male.
J.Natl.Cancer Inst. 1969. 43: 1119.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 20%
subculture procedure - optimal population density 3.0-4.0õ105 cells/ml
cryoconservation - growth medium, 10% DMSO, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 75% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 43-48 chromosomes, modal number
of chromosomes 45, number of markers - 1 is in all cells; the most cells have an attenuation
chromosome number 1 and secondary constriction in chromosome number 16 (routine dye) (ATCC).
Plating efficiency: the cells cannot be plated (ATCC)
virus susceptibility: poliovirus 1; vesicular stomatitis (Indiana).
IgM secretion (lambda light chain).
Isoenzymes G6PD, B.
Erythrocyte rosette tests: E, 0; EA, 0; EAC, 19%.
HLA cell line phenotype A2, Aw33, B7, B14.
Positive for EBNA
Applications: immunology, biochemistry, cell biology.
Collections: ATCC CCL 156; ECACC 85112106; SPBIC.