Origin: human, rhabdomyosarcoma, clone of RD.
Submitted by EVIRI, 1992.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - BS 5%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:4, optimal population density 1.2x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.5 х106 cells/ml in ampule
Viability after cryoconservation: 75% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=46, variability in the range between 42-80 chromosomes, modal number of chromosomes 48
Plating efficiency: 85%
virus susceptibility: CMV; HSV.