RD-Tv K-92

Origin: human, rhabdomyosarcoma, clone of RD.

Submitted by EVIRI, 1992.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - BS 5%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:4, optimal population density 1.2x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.5 х106 cells/ml in ampule

Viability after cryoconservation: 75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 42-80 chromosomes, modal number of chromosomes 48

Plating efficiency: 85%

Other properties:

virus susceptibility: CMV; HSV.

Applications: virology.

Collections: ESCC

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