NAMALVA

Origin: human, Burkitt lymphoma.

Cancer 1969. 23: 64-79; Int.J.Cancer 1972. 10: 44-57; Int.J.Cancer 1973. 12: 396-408;

Antimicrob. Agents Chemother. 1979. 15: 420; Mamaeva S.E. Cell Culture Methods. L.,

Nauka. 1988: 78-98.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS or BS 10%

subculture procedure - optimal population density 3.0-9.0105 cells/ml

cryoconservation - growth medium (may add 40% FBS or BS), 5-10% DMSO, 5.0106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymologycal (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 36-48 chromosomes modal number

of chromosomes 47, number of markers - 12 (differential dye, G-bandig), number of polyploid cells 2.8%.

Other properties:

virus susceptibility: vesicular stomatitis, Sendai.

Secretion of monoclonal antibody (Ig M, lambda light chain).

Support replication of Semliki Forest virus.

Applications: biotechnology (interferon a production), virology, cell biology.

Collections: ATCC CRL 1432; ECACC 87060801; DSM (ACC 24); SPBII; MWIIW; SPBIC.

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