MNNG-HOS (TE 85, clon F-5)

Origin: human, osteosarcoma, chemically transformed (MNNG 0.1 m /ml)

Nature 1975. 256: 51; Int.J.Cancer 1977. 19: 505.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:4 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation: 98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 63-74 chromosomes, modal number

of chromosomes 69-70 , number of polyploid cells 2.2%.

Tumorigenicity: tumorigenic in nude mice

Applications: tumorigenicity, transformation

Collections: ATCC CRL 1547; ECACC 87070201; SPBIC.

Up