MIA PaCa-2

Origin: human, pancreatic carcinoma

Int.J.Cancer 1977. 19: 128-135.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 12.5% or FBS 10%+HS 2.5%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:3 - 1:8, optimal population density 2.0-3.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 5.0106 cells/ml in ampule

 

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, modal number of chromosomes 61 ,

 number of markers - 16-20 (differential dye) (ATCC)

Other properties:

isoenzymes G6PD, B.

Sensitive to asparaginase

Applications: tumorigenicity, enzymology, cell biology

Collections: ATCC CRL 1420; ECACC 85062806; SPBIC.

 

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