M-Hela clone 11

Origin: human, epithelioid cervical carcinoma, strain of Hela, clone of M - Hela

J.Exp.Med. 1953, 97: 695; Cytology (Russ) 1986, 28: 56 - 61

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -

serum - FBS 10%

other components - NEAA 1%

subculture procedure - cells detachment using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio1:5 - 1:10, optimal population density 2.0-4.0x104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation: 79% (0 passage, dye trypan blue)

Sterility: bacteria, fungi and mycoplasma were negative

Species specificity: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46 , variability in the range between 49-50 chromosomes, modal number

of chromosomes 50, number of markers - 13 (differential dye)

Plating efficiency: 60%

Applications: cell biology, tumorigenicity, virology

Collections: SPBIC.

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