LECH-4 (81)

Origin: human, embryo, lung.

Submitted by Glinskikh N.P. et al., Patent N 1147748, 27.01.83.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:2, optimal population density 1.4-1.6x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.5-2.0106 cells/ml in ampule

Viability after cryoconservation: 70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 44-49 chromosomes, modal number of chromosomes 46.

Plating efficiency: 65%

Other properties:

virus susceptibility: polioviruses 1, 2, 3; Coxsackie B3; ECHO 3, 6, 11, 13, 19, 20, 24, 28; RSV,

herpes simplex.

Finite lifetime culture, cells are capable of attaining 49-50 population doubling before onset

of the decline in proliferation.

Applications: virology, biotechnology.

Collections: ESCC

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