Origin: human, embryo, lung.
Submitted by Glinskikh N.P. et al., Patent N 1147748, 27.01.83.
Mode of cultivation: monolayer
Conditions for cultivation: medium - 199/EMEM (1:1)
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:2, optimal population density 1.4-1.6x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.5-2.0õ106 cells/ml in ampule
Viability after cryoconservation: 70% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 44-49 chromosomes, modal number of chromosomes 46.
Plating efficiency: 65%
virus susceptibility: polioviruses 1, 2, 3; Coxsackie B3; ECHO 3, 6, 11, 13, 19, 20, 24, 28; RSV,
Finite lifetime culture, cells are capable of attaining 49-50 population doubling before onset
of the decline in proliferation.
Applications: virology, biotechnology.