L-41 KD/84

Origin: human, monocytic leukemia, clone of J-96 cell line.

Submitted by Glinskikh N.P. et al., EVIRI, patent N 1331061 26.11.85

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3-1:5, optimal population density 1.1-1.5x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.5106 cells/ml in ampule

Viability after cryoconservation: 85-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 42-82 chromosomes, modal number of chromosomes 58.

Plating efficiency: 65%

Other properties:

virus susceptibility: polioviruses; Coxsackie B; ECHO-19; human adenoviruses; measles.

Applications: virology.

Collections: ESCC

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