Origin: human, monocytic leukemia, clone of J-96 cell line.
Submitted by Glinskikh N.P. et al., EVIRI, patent N 1331061 26.11.85
Mode of cultivation: monolayer
Conditions for cultivation: medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3-1:5, optimal population density 1.1-1.5x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.5õ106 cells/ml in ampule
Viability after cryoconservation: 85-90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 42-82 chromosomes, modal number of chromosomes 58.
Plating efficiency: 65%
virus susceptibility: polioviruses; Coxsackie B; ECHO-19; human adenoviruses; measles.