Hs 578 T
Origin: human, ductal breast carcinoma
J.Natl.Cancer Inst. 1977. 58: 1795-1806.
Morphology: epithelial-like, multi-layered
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM
serum - FBS 10%
other components - bovine insulin 10m g/ml
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:3 - 1:6,
optimal population density 2.0-4.0x104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46 , variability in the range between 50-77 chromosomes, modal number of chromosomes 59,
number of markers - 10 (differential dye, ATCC), number of polyploid cells 15.8%.
Tumorigenicity: tumorigenic in immunosupressed mice
estrogen receptors were not detected.
Isoenzymes G6PD, B; PGM1,1; PGM3,1; ES D,1; Me-2, 0; AK 1,1; GLO-1,1.
Applications: antitumor tests, radiotherapy, tumorigenicity:
Collections: ATCC HTB 126; ECACC 86082104; SPBIC.