Hs 578 T

Origin: human, ductal breast carcinoma

J.Natl.Cancer Inst. 1977. 58: 1795-1806.

Morphology: epithelial-like, multi-layered

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 10%

other components - bovine insulin 10m g/ml

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:3 - 1:6,

optimal population density 2.0-4.0x104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46 , variability in the range between 50-77 chromosomes, modal number of chromosomes 59,

number of markers - 10 (differential dye, ATCC), number of polyploid cells 15.8%.

Tumorigenicity: tumorigenic in immunosupressed mice

Other properties:

estrogen receptors were not detected.

Isoenzymes G6PD, B; PGM1,1; PGM3,1; ES D,1; Me-2, 0; AK 1,1; GLO-1,1.

Applications: antitumor tests, radiotherapy, tumorigenicity:

Collections: ATCC HTB 126; ECACC 86082104; SPBIC.