Hos (TE85, clone F5)

Origin: human, osteosarcoma.

Cancer 1971. 27: 397-402; Int.J.Cancer 1975. 15: 23-29; Int.J.Cancer 1975. 16: 840-849.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum - FBS 10%

other components - NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:2 - 1:6,

 optimal population density 2.0-4.0x104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 2.5106 cells/ml in ampule

Viability after cryoconservation: 95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, modal number of chromosomes 49, number of polyploid cells 3.6%.

Other properties:

cells are sensitive to both virus and chemical transformation

Applications: virology, transformation, biochemistry

Collections: ATCC CRL 1543; ECACC 87070202; MWIIW; SPBIC.

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