Hela gniiem

Origin: human, epithelioid cervical carcinoma, clone of Hela.

Cancer Res. 1952. 12: 264.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:5 -1:6, optimal population density 0.8 -1.2 x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.0 106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 42-70 chromosomes,

 modal number of chromosomes 61-65.

Plating efficiency: 60 %

Other properties:

virus susceptibility:Coxsackie A, B1, B3, B5; ECHO; human adenoviruses 1-7.

Presence of oncovirus B.

Applications: virology.

Collections: ESCC