Origin: human, epithelioid cervical carcinoma, clone of Hela.
Cancer Res. 1952. 12: 264.
Mode of cultivation: monolayer
Conditions for cultivation: medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:5 -1:6, optimal population density 0.8 -1.2 x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.0 õ106 cells/ml in ampule
Viability after cryoconservation: 80% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 42-70 chromosomes,
modal number of chromosomes 61-65.
Plating efficiency: 60 %
virus susceptibility:Coxsackie A, B1, B3, B5; ECHO; human adenoviruses 1-7.
Presence of oncovirus B.