Origin: human, epithelioid cervical carcinoma, strain of Hela. Submitted from Free University of Brussels, Belgium
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM or DMEM
serum - FBS 10%
other components - NEAA 1% (EMEM)
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3 - 1:5 , optimal population density 1.0-5.0x104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.5х106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n=46 , variability in the range between 57-60 chromosomes, modal number of chromosomes 59,
markers - 25 (differential dye)
deficient in thymidine kinase, resistant to 5-bromodeoxyuridine.
Applications: somatic cell genetics, cell biology
Collections: : SPBIC.