Hela TK-

Origin: human, epithelioid cervical carcinoma, strain of Hela. Submitted from Free University of Brussels, Belgium

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS 10%

other components - NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:3 - 1:5 , optimal population density 1.0-5.0x104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.5х106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46 , variability in the range between 57-60 chromosomes, modal number of chromosomes 59,

 markers - 25 (differential dye)

Other properties:

deficient in thymidine kinase, resistant to 5-bromodeoxyuridine.

Applications: somatic cell genetics, cell biology

Collections: : SPBIC.