Hela-D

Origin: human, epithelioid cervical carcinoma, clone of Hela

Cancer Res. 1952. 12. 264. Submitted by Glinskikh N.P. et al., EVIRI, patent N 1036052 15.04.89

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:4-1:6,

 optimal population density 1.0-1.3x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0106 cells/ml in ampule

Viability after cryoconservation: 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 46-71 chromosomes, modal number of chromosomes 61-65.

Plating efficiency: 70%

Other properties:

virus susceptibility: polioviruses; Coxsackie B1, B3, B5; ECHO; human adenovirus 7.

Presence of oncoviruses A and B

Applications: virology, biotechnology.

Collections: ESCC

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