Origin: human, epithelioid cervical carcinoma, clone of Hela
Cancer Res. 1952. 12. 264. Submitted by Glinskikh N.P. et al., EVIRI, patent N 1036052 15.04.89
Mode of cultivation: monolayer
Conditions for cultivation: medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:4-1:6,
optimal population density 1.0-1.3x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 2.0õ106 cells/ml in ampule
Viability after cryoconservation: 80 % (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 46-71 chromosomes, modal number of chromosomes 61-65.
Plating efficiency: 70%
virus susceptibility: polioviruses; Coxsackie B1, B3, B5; ECHO; human adenovirus 7.
Presence of oncoviruses A and B
Applications: virology, biotechnology.