Origin: human, fibrosarcoma.

Cancer 1974. 33: 1027-1033.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS 10%

other components -

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

 split ratio 1:4 - 1:8, optimal population density 1.0-4.0104 cells/cm2

cryoconservation - growth medium, 5%DMSO, 1.2106 cells/ml in ampule

Viability after cryoconservation: 96% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: isoenzymological (LDH, G6PD) analysis (LDH and G6PD)

Karyology: 2n= 46, variability in the range between 44-48 chromosomes, modal number

of chromosomes 46, pseudodiploid, about 40% of the cells had rearranged karyotypes (ATCC)

Plating efficiency: 3% (ATCC)

Tumorigenicity: tumorigenic in NIH Swiss mice immunosupressed with anti-thymocytic serum -.

Other properties:

virus susceptibility: - RNA tumor viruses (RD 114, FelV), poliovirus 1, vesic. stomatitis (Indiana).

Isoenzymes G6PD, B.

Chemotaxis, chemoinvasion, matrigel invasion.

Collagen production

Applications: molecular and cell biology, cytotoxicity, tumorigenicity, virology.

Collections: ATCC CCL 121; ECACC 85111505; SPBIC.