Origin: human, fibrosarcoma.
Cancer 1974. 33: 1027-1033.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM or DMEM
serum - FBS 10%
other components -
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),
split ratio 1:4 - 1:8, optimal population density 1.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 5%DMSO, 1.2õ106 cells/ml in ampule
Viability after cryoconservation: 96% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: isoenzymological (LDH, G6PD) analysis (LDH and G6PD)
Karyology: 2n= 46, variability in the range between 44-48 chromosomes, modal number
of chromosomes 46, pseudodiploid, about 40% of the cells had rearranged karyotypes (ATCC)
Plating efficiency: 3% (ATCC)
Tumorigenicity: tumorigenic in NIH Swiss mice immunosupressed with anti-thymocytic serum -.
virus susceptibility: - RNA tumor viruses (RD 114, FelV), poliovirus 1, vesic. stomatitis (Indiana).
Isoenzymes G6PD, B.
Chemotaxis, chemoinvasion, matrigel invasion.
Applications: molecular and cell biology, cytotoxicity, tumorigenicity, virology.
Collections: ATCC CCL 121; ECACC 85111505; SPBIC.