Origin: human, epidermoid larynx carcinoma, clone of HEp-2
Submitted by Glinskikh N.P. et al., EVIRI, patent N 1122693 27.09.83.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM/lactalbumin hydrolysate (1:1)
serum - BS 5%
subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:4-1:6,
optimal population density 0.7 -1.2 x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 2.0 õ106 cells/ml in ampule.
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 54-68 chromosomes,
modal number of chromosomes 56.
Plating efficiency: 60%
virus susceptibility: adenoviruses; RSV, ECHO; Coxsackie B1, B3; polioviruses.
Presence of oncornaviruses A and B