HEp-2- D

Origin: human, epidermoid larynx carcinoma, clone of HEp-2

Submitted by Glinskikh N.P. et al., EVIRI, patent N 1122693 27.09.83.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM/lactalbumin hydrolysate (1:1)

serum - BS 5%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:4-1:6,

optimal population density 0.7 -1.2 x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0 106 cells/ml in ampule.

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 54-68 chromosomes,

modal number of chromosomes 56.

Plating efficiency: 60%

Other properties:

virus susceptibility: adenoviruses; RSV, ECHO; Coxsackie B1, B3; polioviruses.

Presence of oncornaviruses A and B

Applications: virology.

Collections: ESCC