HBL-100

Origin: human, breast, epithelial cells

In Vitro 1980. 16: 211; J.Natl.Cancer Inst. 1982. 69: 483-487; Exp.Cell Res. 1985. 160: 83-94.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - Mc Coy's 5A or RPMI 1640

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:2 - 1:4

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 52-77 chromosomes,

 modal number of chromosomes 66-68,

markers - 28 (differential dye, ATCC).

Tumorigenicity: tumorigenic in nude mice

Other properties:

isoenzymes G6PD, B; PGM1,1; PGM3,2; ES D,1; Me-2,0; GLO-1,2; AK 1,1-2.

Tandemly integr. SV-40 virus genome.

HLA cell line phenotype A1, 10-11; B7, 8.

Applications: virology, tumorigenicity, genetics

Collections: ATCC HTB 124; SPBII; SPBIC.

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