Origin: human, heart, atrial appendage
J.Natl.Cancer Inst. 1964. 33: 285; Fed. Proc. 1975. 16: 14.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME or EMEM
serum - FBS 10%
other components - NEAA 1% (ΕΜΕΜ)
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:5
cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 85% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n=46 , variability in the range between 60-73 chromosomes, modal number of chromosomes 69,
number of markers - 1 large submetacentric chromosome, number of poliploid cells - 12.8%.
Plating efficiency: 60% (ATCC)
Tumorigenicity: produce tumors in the cheek pouch of the Syrian hamster.
virus susceptibility poliovirus, Coxsackie A 11, 13, 15 and 18; Coxsackie B group; ECHO; ECM; NDV; RS;
mumps; parainfluenza 2; adenovirus 3, 4 and 7; herpes; vaccinia.
Collections: ATCC CCL 27; SPBIC.