Girardi Heart

Origin: human, heart, atrial appendage

J.Natl.Cancer Inst. 1964. 33: 285; Fed. Proc. 1975. 16: 14.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME or EMEM

serum - FBS 10%

other components - NEAA 1% (ΕΜΕΜ)

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:5

cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46 , variability in the range between 60-73 chromosomes, modal number of chromosomes 69,

number of markers - 1 large submetacentric chromosome, number of poliploid cells - 12.8%.

Plating efficiency: 60% (ATCC)

Tumorigenicity: produce tumors in the cheek pouch of the Syrian hamster.

Other properties:

virus susceptibility poliovirus, Coxsackie A 11, 13, 15 and 18; Coxsackie B group; ECHO; ECM; NDV; RS;

mumps; parainfluenza 2; adenovirus 3, 4 and 7; herpes; vaccinia.

Applications: virology

Collections: ATCC CCL 27; SPBIC.

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